The human impact and weather conditions alter the microbial composition in the environment. A winter sampling of seashore sand was achieved in two touristic points in the Golf of Naples obtaining 3 bioactive strains with antimicrobial activity in vitro against human pathogens. The 16S rRNA sequence shows the closest similarity with Halobacillus sp., Jeotgalibacillus sp. and Catenovulum sp. and the genome of the closest members related reveals the presence of Biosyntehtic Gene Clusters (BGC) with less than 50% and unknown similarity with known compounds.