Below are a number of sequencing facilities that can provide Sanger sequencing services for your Tiny Earth course. In contacting companies/facilities, we learned that many institutions are ending their Sanger sequencing services. We’ve been able to identify the facilities below and in some cases work out deals that can help reduce costs. Links and quote information is provided below. In some cases you may find that your institution already has a deal with some of these companies that might be cheaper than the quote I was able to obtain (for example, institutions close to major sequencing hubs (i.e. San Diego or San Jose area) are able to get more competitive pricing).
Please send us feedback as you use them regarding your experience and data quality or contact me if you have additional resources we can include.
Yale had been one of our go to sequencing facilities, but this year had to begin charging users external rates, which led to a substantial increase in prices. While higher, there can be a benefit of a facility with whom you have an established relationship. Also the Assistant Director, Carol Mariani, is very helpful with feedback about your samples and has indicated she is more than happy to help with troubleshooting and feedback for users. Such availability can be harder to come by at some commercial facilities. To set up new account: https://dna-analysis.yale.edu/new-user-account-request
Customer sends purified PCR products ready for sequencing
96-well Plate $255/plate
Yale will purify PCR products and proceed with standard sequencing service
96-well Plates $335/plate
The price agreement linked to Tiny Earth is 3135497. Use the portal at https://b2b.eurofinsgenomics.com/custom/tinyearth. Use this quote number when submitting samples or when contacting representative Jessica Rhine.
Orders over $30 qualify for free shipping, but it is currently not available through the custom portal link above. In the meantime, you can order the free shipment kits on the portal which come with the prepaid UPS labels to cover shipping. Primers can be sent in larger quantities that they will keep for future sequencing runs.
96 well Plate $254.40/plate
96 well Plates $350.40/plate
Note: I don’t know people who have used before, please report back on quality and experience if you use.
Quote for Tiny Earth Classes: CM100318-279 (this should be written across the order sheet that will be sent in with samples. If PCR clean-up is requested this should also be written at top of the order sheet. It is recommended that customers ship by FedEx overnight (not needed to send on ice). Wrap samples in parafilm. Need 10 ul volume of sample. Primers should be sent in separate tube (not pre-mixed) at concentration of 5 pmol/ul, they want 2 ul per reaction.
- $0.75-1 for clean-up
- Fewer than 48 samples send in microcentrifuge tubes (1.5 ml)
- More than 48 samples, send in sealed plate
Great Lakes Genomics Center
UW System Institutions may want to try this resource, as they have discounted prices for UW institutions (below is the UW internal rate). However, when discussing with them they indicated they may be discontinuing Sanger sequencing in the near future.
96-well plate $350.00/plate
24-95 samples $3.65/reaction
1-24 samples $5.00/reaction
Quotation # 7295 (see accompanying sheet)
1 – 95 Reactions: $4.00 /Reaction
96 + Reactions: $3.50 /Reaction
1 – 95 Samples: $1.00 /Sample
96 + Samples: $0.75 /Sample
(via Lisa Grubisha UW-Green Bay)
This may be one of the least expensive options if you are doing 48 sequences or more.Although the pricing scheme is a little difficult to navigate and while the 94 sample price is what Lisa indicates she pays, they do indicate that external rates could be 10% higher.
Sequencing reaction setup, cleanup and sequencing, 1-20 samples, $9.94
Sequencing reaction setup, cleanup and sequencing, 21-60 samples, $162.83
Flat price for all units (range of $3.39/sample beginning at 48 samples, to 2.71/sample at 60 samples)
Sequencing reaction setup, cleanup and sequencing, 61-94 samples, $195.00
Flat price/plate/per reaction (price range of $3.19 (61 samples) to $2.07/sample (94 samples)