Below are a number of sequencing facilities that can provide Sanger sequencing
services for your Tiny Earth course. In contacting companies/facilities, we learned that
many institutions are ending their Sanger sequencing services. We’ve been able to
identify the facilities below and in some cases work out deals that can help reduce costs.
Links and quote information is provided below. In some cases you may find that your
institution already has a deal with some of these companies that might be cheaper than
the quote I was able to obtain (for example, institutions close to major sequencing hubs
(i.e. San Diego or San Jose area) are able to get more competitive pricing).
Please send us feedback as you use them regarding your experience and data quality or contact me if you have additional resources we can include.
Yale had been one of our go to sequencing facilities, but this year had to begin charging
users external rates, which led to a substantial increase in prices. While higher, there can
be a benefit of a facility with whom you have an established relationship. Also the
Assistant Director, Carol Mariani, is very helpful with feedback about your samples and
has indicated she is more than happy to help with troubleshooting and feedback for
users. Such availability can be harder to come by at some commercial facilities.
Full Service– Yale will purify PCR products and proceed with standard sequencing
96-well Plates $335/plate
Standard Service– customer sends purified PCR products ready for sequencing
96-well Plate $255/plate
(to set up new account: https://dna-analysis.yale.edu/new-user-account-request)
$4/Tube reaction; if requested, PCR clean-up is additional $2/ tube
$2.65/Plate reaction ($254.40 per 96); if requested, PCR clean-up is additional $96 per
plate (total $350.40 per plate).
Customers can create an account at the link below that will automatically be associated
with discounted pricing. The price agreement linked to Tiny Earth is 3135497 and you
should use this quote number when submitting samples (if not through portal) or when
contacting representative- firstname.lastname@example.org.
Orders over $30 qualify for free shipping, but it is currently not available through the
custom portal link above. In the meantime, you can order the free shipment kits on the
portal which come with the prepaid UPS labels to cover shipping. Primers can be sent in
larger quantities that they will keep for future sequencing runs.
Note: I don’t know people who have used before, please report back on quality and
experience if you use.
$0.75-1 for clean-up
Fewer than 48 samples send in microcentrifuge tubes (1.5 ml)
More than 48 samples, send in sealed plate.
Quote for Tiny Earth Classes: CM100318-279 (this should be written across the order
sheet that will be sent in with samples. If PCR clean-up is requested this should also be
written at top of the order sheet.
It is recommended that customers ship by FedEx overnight (not needed to send on ice).
Wrap samples in parafilm. Need 10 ul volume of sample. Primers should be sent in
separate tube (not pre-mixed) at concentration of 5 pmol/ul, they want 2 ul per reaction.
UW System Institutions may want to try this resource, as they have discounted prices for
UW institutions (below is the UW internal rate). However, when discussing with them
they indicated they may be discontinuing Sanger sequencing in the near future.
96-well plate $350.00/plate
24-95 samples $3.65/reacSon
1-24 samples $5.00/reacSon
Quotation # 7295 (see accompanying sheet)
1 – 95 Reactions: $4.00 /Reaction
96 + Reactions: $3.50 /Reaction
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1 – 95 Samples: $1.00 /Sample
96 + Samples: $0.75 /Sample
University of Kentucky Genomics Core Laboratory (via Lisa Grubisha UW-Green Bay)
This may be one of the least expensive options if you are doing 48 sequences or more.
Although the pricing scheme is a little difficult to navigate and while the 94 sample price
is what Lisa indicates she pays, they do indicate that external rates could be 10% higher.
RES SANGER SEQUENCIN TIER 3.1, Sequencing reaction setup, cleanup and
sequencing, 1-20 samples, $9.94, Per sample
RES SANGER SEQUENCING TIER 3.3, Sequencing reaction setup, cleanup and
sequencing, 21-60 samples, $162.83, Flat price for all units (range of $3.39/sample
beginning at 48 samples, to 2.71/sample at 60 samples)
RES SANGER SEQUENCING TIER 3.4, Sequencing reaction setup, cleanup and
sequencing, 61-94 samples, $195.00, Flat price / plate / per reaction (price range of
$3.19 (61 samples) to $2.07/sample (94 samples)).