With the severity of antibiotic resistant bacteria on the rise, it is critical that discovery of new antimicrobial activity is done to combat these dangerous microbes. The use of antibiotics have been a pivotal step towards advances in both human and veterinary medicine. Through the years, strains of bacteria have evolved to combat different antimicrobial mechanisms used to destroy bacteria which endanger the efficacy of current antibiotic drugs. New antibiotics not only can be developed synthetically, but they also can be discovered naturally in the environment. In attempts to increase their fitness, bacteria develop antibacterial properties to survive. It is by isolating these bacteria that exhibit antimicrobial success on resistant bacteria that new antibiotics are discovered. This research is in accordance with the Small World Initiative’s mission for researchers to discover antibiotics from soil isolates at the University of Dayton.