17. The Isolation and Analysis of Antibiotics from Bacteria Found in Rabbit Feces

The Isolation and Analysis of Antibiotics from Bacteria Found in Rabbit Feces

Kayla Petry, Joanna Klein
University of Northwestern – St. Paul

Two bacterial isolates that have inhibitory properties against ESKAPE safe-relatives of the ESKAPE pathogens had previously been identified from rabbit feces. One isolate was from the genus Panibacillus and the other was from the genus Bacillus. The goal of this research was to isolate the antibiotics these two bacteria produce. First, we sought to adapt a random transposon mutagenesis protocol found to work for many Pseudomonas isolates to identify the biosynthetic gene clusters responsible for the inhibition phenotype. Biochemical extraction of active compounds was performed using ethyl acetate to isolate the organic compounds which had antibiotic properties. The organic compounds that were extracted inhibited the growth of Bacillus subtilis. The compounds were further analyzed to see if they had any effect of eukaryotic organisms, and both were found to inhibit the germination and growth of chia seeds