Antibiotic resistance is a prominent problem and research is being done to find solutions to combat serious pathogens that can be deadly to humans and our crops. Research has found that certain antibiotics found in soil can be useful in fighting these resistant pathogens. Therefore, this experiment focuses on collecting soil and screening them to find more antibiotic producers. Additionally, the experiment analyzes the effects of NaNO3 treated soil on the likelihood of discovering antibiotic products. As a result, there was no statistically significant difference found in the amount of producers in untreated soil and NaNO3 treated soil. The experiment still produced discoveries of potentially helpful antibiotic producers and for further insight, more trials of the experiment could lead to a statistically significant result.