23. Antibiotic Producing Bacteria from Sacramento State University Campus Soil

Antibiotic Producing Bacteria from Sacramento State University Campus Soil

Nguyen Vo, Linda Acevedo, Elizabeth Kern, Enid Gonzalez-Orta Ph.D.
California State University, Sacramento

In this research, we focused on the Safe ESKAPE relatives with the same physiological features as the ESKAPE pathogens that exhibit multidrug resistance responsible for the antibiotic resistance crisis. We collected the soil sample near the river, right off the bike and walking path at Sacramento State University. We tested nine isolates against five different Safe ESKAPE relatives and cultured them on R2A media. Only five isolates exhibited antibiotic activity. Four of the isolates inhibited Gram-positive bacteria, including S. epidermis, E. raffinosus, and B. subtilis. Only one of the isolates inhibited Gram-negative bacteria E. coli. We performed biochemical tests, DNA Extraction, Polymerase Chain Reaction, Gel Electrophoresis, and Nanodrop on the isolates. We also analyzed the bacteria sequences through different bioinformatic programs, including BLAST and the Ribosomal Database Project (RDP) [SeqMatch and Classifier]. Using these programs was determined that the five isolates chosen belonged to the genera Bacillus, Streptomyces, and Flavobacterium.