Tiny Earth Protocols
Tiny Earth Chemistry Hub (TECH)
University of Wisconsin–Madison
Dr. Angelo Kolokithias
Northeast Wisconsin Technical College
Soil Sampling
Labeling and Spreading Plates
Making a Slant
Catalase Test
Gram Stain
Typical Media Menu – Making Media
ESKAPE Screening – Spread/Patch
Inoculating a Stab/Fermentation Test
Agarose Gel Electrophoresis
Serial Dilutions
Pick and Patch
Streak Plate
Making an Overnight Culture
ESKAPE Screening – Patch/Patch
ESKAPE Screening – Top Agar
Glycerol Stock Preparation
PCR Analysis